Applications
Discover Monolith applications notes, technical notes and protocols
Application and Technical notes
- • Analyzing the binding affinity of aptamer quantum dot conjugates to VEGF
- • Binding of Calcium Ions to Synaptotagmin
- • Binding of Histone peptides to Chromatin assembly factor I (CAF-I) p48 subunit
- • Binding of the geldanamycin derivative 17-DMAG to Hsp90
- • Competitive Assay Approach: Binding of Small Molecules to the Active Form of p38
Protocols: protein - small molecule interactions
- • ALDH21 – NADP+ protocol
- • ATP Binding Cassette Transporter – Phosphite protocol
- • Bovine Serum Albumin – Ibuprofen protocol
- • Bovine Serum Albumin – TWEEN® 20 protocol
- • Bovine Serum Albumin – Zinc protocol
Protocols: protein - protein interactions
- • 30S Ribosomal Subunit – YjeQ protocols
- • Amyloid-Beta – Protein X protocol
- • B. burgdorferi CspA – C9 protocol
- • Beta-Amylase – Serpin-Z4 protocol
- • Bovine Serum Albumin Self-Oligomerization protocol
Protocols: ternary interactions
Protocols: nucleic acid interactions
- • BoxP1 Hairpin DNA – Mlc protocol
- • DNA Aptamer – AMP (C6 amino) (with lysine labeling) protocol
- • DNA Aptamer – AMP (displacement assay) protocol
- • DNA Aptamer – AMP protocol
- • DNA Aptamer – Ochratoxin (in milk) protocol
Protocols: antibody interactions
- • IgG – FcRn protocol
- • Anti-CD42b – CD42b (on intact platelets) protocols
- • Affibody – IgM-Fc (stoichiometry) protocol
- • Protein A – Rabbit & Mouse IgG protocol
- • Protein A – Herceptin (with cystine labeling) protocol
Protocols: protein - carbohydrate interactions
- • CMB3a – Cellulose Nanocrystals protocol
- • Lysozyme – NAG3 (biotinylated) protocol
- • Lysozyme – NAG3 (label-free) protocol
- • Lysozyme – NAG3 (no column purification) protocol
- • Lysozyme – NAG3 (thermodynamics) protocol
Protocols: miscellaneous interactions
- • Bicelles – Dystrophin (R1-3) protocol
- • Critical Micelle Concentration (CMC) of LMNG protocol
- • Influenza Virus X31 – Inhibitor Coated Nanoparticle protocol
- • Reversible Quenching of Red Fluorophores by TCEP protocol
- • Size exclusion chromatography of membrane proteins to remove free dye captured by micelles protocol