Applications
Discover Monolith applications notes, technical notes and protocols
Application and Technical notes
- • Analyzing the binding affinity of aptamer quantum dot conjugates to VEGF
- • Binding of calcium ions to synaptotagmin
- • Binding of Histone peptides to chromatin assembly factor I (CAF-I) p48 subunit
- • Binding of the geldanamycin derivative 17-DMAG to Hsp90
- • Competitive assay approach: binding of small molecules to the active form of p38
Protocols: protein - small molecule interactions
- • ALDH21 – NADP+ protocol
- • ATP binding cassette transporter – phosphite protocol
- • Bovine Serum Albumin – Ibuprofen protocol
- • Bovine serum albumin – TWEEN® 20 protocol
- • Bovine serum albumin – zinc protocol
Protocols: protein - protein interactions
- • 30S ribosomal subunit – YjeQ protocol
- • Amyloid-beta – protein X protocol
- • B. burgdorferi CspA – C9 protocol
- • Beta-amylase – serpin-Z4 protocol
- • Bovine serum albumin self-oligomerization protocol
Protocols: ternary interactions
Protocols: nucleic acid interactions
- • BoxP1 Hairpin DNA – Mlc protocol
- • BoxP1 hairpin DNA – Mlc protocol
- • DNA aptamer – AMP (C6 amino) (with lysine labeling) protocol
- • DNA aptamer – AMP (displacement assay) protocol
- • DNA Aptamer – AMP (thermodynamics) protocol
Protocols: antibody interactions
- • HER2 – Herceptin ADCs
- • Anti-6x-His Tag Monoclonal Antibody – p38-alpha protocol
- • Herceptin – HER2 protocol
- • IgG – FcRn protocol
- • Anti-CD42b – CD42b (on intact platelets) protocols
Protocols: protein - carbohydrate interactions
- • CMB3a – cellulose nanocrystals protocol
- • Lysozyme – NAG3 (biotinylated) protocol
- • Lysozyme – NAG3 (label-free) protocol
- • Lysozyme – NAG3 (no column purification) protocol
- • Lysozyme – NAG3 (spectral shift) protocol
Protocols: miscellaneous interactions
- • Bicelles – dystrophin (R1-3) protocol
- • Critical micelle concentration (CMC) of LMNG protocol
- • Influenza virus X31 – inhibitor coated nanoparticle protocol
- • Reversible quenching of red fluorophores by TCEP protocol
- • Size exclusion chromatography of membrane proteins to remove free dye captured by micelles protocol