The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. His tag-specific antibodies are used to facilitate detection or immunoprecipitation of His-tagged proteins. In this protocol, the interaction between the anti-His6 antibody HIS.H8 and His6-tagged mitogen-activated protein kinase 14 (p38-α) is measured.

protein – protein | antibody | MOX-P-116