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ALDH21 – NADP+ protocol

Aldehyde dehydrogenases constitute a superfamily of NAD(P)+-dependent enzymes, which detoxify aldehydes produced in various metabolic pathways. Plants comprise 13 ALDH families, five of them, ALDH11, ALDH19, ALH21, ALDH22 and ALDH23, unique to plants. ALDH21 from the moss P. patens codes for a tetrameric NADP+-dependent succinic semialdehyde dehydrogenase (SSALDH), which converts succinic semialdehyde, an intermediate of the c-aminobutyric acid (GABA) shunt pathway, into succinate in the cytosol. Lower plant species including some green algae, non-vascular plants (bryophytes) as well as the oldest vascular plants (lycopods) and ferns (monilophytes) possess a unique aldehyde dehydrogenase (ALDH) gene named ALDH21. However, the gene is absent in flowering plants. The expression of ALDH21 gene is strongly induced by a desiccation and turns back upon rehydration in the twisted mosses S. ruralis and S. caninervis. Also, the overexpression of ALDH21 in cotton and tobacco improves their drought and salt tolerance.

protein – small molecule interaction | plant biology | ALDH | MO-P-056

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