Binding of the geldanamycin derivative 17-DMAG to Hsp90
Abstract
For proper folding, many proteins involved in signal-transduction pathways, cell-cycle regulation and apoptosis depend upon the ATP-dependent molecular chaperone Hsp90. Consequently Hsp90 turned out to be an attractive target for cancer therapeutics. In this study we demonstrate the binding of the geldanamycin derivative 17-DMAG to Hsp90 using MicroScale Thermophoresis (MST). The study also highlights the high content information of the MST measurements as one important benefit of MicroScale Thermophoresis.