Bovine Serum Albumin – Ibuprofen protocol
Bovine serum albumin (also known as BSA) is a serum albumin protein derived from cows, which is often used as a protein concentration standard in lab experiments. Among its 583 amino acid residues, it contains 17 intrachain disulfide bonds as well as one free thiol group, which can be used for site-specific labeling with a thiol-reactive (maleimide) fluorescent dye. Ibuprofen is a nonsteroidal anti-inflammatory drug that is used for treating pain, fever, and inflammation. It binds to a hydrophobic pocket of BSA (Sudlow site II) which is the principal binding site for drugs.
protein – small molecule interaction | maleimide | MO-P-018