IgG – FcRn protocol
IgG is a significant antibody class found in the blood. In addition to its importance for the immune system, IgG subclasses also display a remarkable persistence with a half-life of 20-23 days, except IgG3 that has a half-life of just 7 days. The extended half-life is due to the large MW of IgGs and to their interactions with the neonatal Fc receptor (FcRn). FcRn is an MHC class I-like molecule that consists of a heavy transmembrane chain (HC) that non-covalently associates with β2-microglobulin. The IgG and FcRn interaction occurs in a strictly pH-dependent manner with negligible binding and release in neutral pH. In this protocol the binding between human IgG1 and the IgG receptor (FcRn) was investigated.
antibody – receptor interaction | IgG | FcRn | pharmacokinetics | IgG half-life | MO-P-074