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p38-alpha – SB 203580 (competitive assay) protocol

Mitogen-activated protein kinase 14 (also called p38-α) is an enzyme that has been implicated in the regulation of many proinflammatory pathways. It is a drug target for many diseases such as rheumatoid arthritis, endotoxic shock and osteoporosis. SB 203580 is a specific and potent inhibitor of kinases that binds p38-α with nM affinity. Compounds that bind to the ATP site or to an allosteric site altering the conformation of the ATP site (Type I and Type II kinase inhibitors) can be detected in a competitive assay using a fluorescent tool compound that is based on ATP-competitive kinase inhibitors.

protein – small molecule interaction | kinase | inhibitor | competitive assay | MO-P-003

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