Anti-CD42b – CD42b (on intact platelets) protocols
The glycoprotein (GP) Ib-IX-V complex is a vital receptor for the initiation of hemostasis as well as thrombosis. It binds to the multimeric adhesive ligand, von Willebrand factor, found in endothelial cells, megakaryocytes, and platelets. It is essential for regulating platelet adhesion under high shear to sub endothelium, endothelial cells or leukocytes, procoagulant complex assembly on the platelet surface and signaling involving activation of integrins. Its absence or deficiency leads to Bernard-Soulier syndrome. GP1b, also called CD42b is a 145 kDa protein that, together with CD42c, forms a heterodimer of 160 kDa consisting of an α-chain and a β-chain. The different subunits are linked together via a disulfide bridge. CD42c forms a non-covalent complex together with CD42a and CD42d in the membrane of the platelet. The CD42 complex is a receptor of von Willebrand factor and thrombin, which mediate thrombocyte adhesion to subendothelial matrixes, which are exposed during endothelial damage.
antibody – receptor interaction | platelets | whole cell interaction | MO-P-050