Assay Optimization
Assay Optimization
- • Adsorption
- • Aggregation
- • Buffer - assay buffer composition
- • Buffer autofluorescence
- • Buffer gradients
- • Control experiment
- • Detergents
- • DMSO
- • Fluorescent fusion proteins
- • Fluorophores
- • Fragment-based drug discovery
- • Freeze thaw cycles
- • Ligand autofluorescence
- • Liquid handling
- • Ligand-induced fluorescence change
- • Ligand-induced photobleaching
- • Mixing
- • PEG 8000
- • Positive control sample
- • Protein concentration and DOL
- • Protein labeling
- • Quenching
- • Random changes in initial fluorescence
- • RED-tris-NTA
- • Reference sample
- • Reducing agents
- • Serial dilution
- • Target and ligand concentrations