A fluorophore is a molecule with Fluorescence properties. This means that the fluorophore absorbs photons and emits photons of lower energy in return. Fluorophores are chemically diverse. Fluorophores relevant in the life sciences range from small chemical compounds (fluorescein, rhodamine, cyanine, and their derivatives) to amino acids (tryptophan, tyrosine, phenylalanine) to fluorescent fusion proteins (GFP, RFP etc.). Chemical fluorophores are typically attached to biomolecules via covalent or tag-specific Protein Labeling procedures, while fluorescent proteins can be genetically fused to the target protein.

NanoTemper Technologies offers several Protein Labeling kits with different coupling chemistries (Covalent and affinity-based) which are optimized for TRIC and Spectral Shift experiments. Please contact the NanoTemper Technologies Support for any further questions about our labeling kits.

Alternatively, when selecting Oligonucleotides or small molecules to use as targets, selecting Cy5-labeled oligonucleotides by synthesis or adding Cy5 to the small molecule during synthesis are great alternatives.

Note: When choosing a fluorophore for your experiment, make sure it matches the Dianthus Filterset. Excitation and emission wavelengths often vary depending on the environment (buffer, pH). All NanoTemper Technologies RED Labeling kits are compatible with Dianthus.

A fluorophore is a molecule with Fluorescence properties. This means that the fluorophore absorbs photons and emits photons of lower energy in return. Fluorophores are chemically diverse. Fluorophores relevant in the life sciences range from small chemical compounds (fluorescein, rhodamine, cyanine, and their derivatives) to amino acids (tryptophan, tyrosine, phenylalanine) to fluorescent fusion proteins (GFP, RFP etc.). Chemical fluorophores are typically attached to biomolecules via covalent or tag-specific Protein Labeling procedures, while fluorescent proteins can be genetically fused to the target protein.

NanoTemper Technologies offers several Protein Labeling kits with different coupling chemistries (Covalent and affinity-based) which are optimized for TRIC and Spectral Shift experiments. Please contact the NanoTemper Technologies Support for any further questions about our labeling kits.

Alternatively, when selecting Oligonucleotides or small molecules to use as targets, selecting Cy5-labeled oligonucleotides by synthesis or adding Cy5 to the small molecule during synthesis are great alternatives.

Note: When choosing a fluorophore for your experiment, make sure it matches the Dianthus Filterset. Excitation and emission wavelengths often vary depending on the environment (buffer, pH). All NanoTemper Technologies RED Labeling kits are compatible with Dianthus.