Repeated freezing and thawing of biomolecules such as proteins, can lead to significant denaturation even in the presence of cryoprotectants, which is why aliquoting protein preparations is generally recommended. Low sample quality, e.g. due to repeated freeze-thaw cycles, can in turn significantly affect reproducibility and quality of downstream characterization steps such as binding assays. It is especially recommended to test if repeated cycles of freezing and thawing of the Target protein affect the result of an interaction measurement. This can be done by comparing the result of a Binding Check experiment for different aliquots.

Especially protein Aggregation is an issue that can be detrimental to data quality, especially for TRIC assays. Knowing protein stability and quality first can therefore save both time and money e.g. in subsequent screening campaigns.