Assay set up

Target labeling

• • Protein labeling
• • Degree-of-labeling (DOL)
• • Properties of NanoTemper technologies dyes

Pre-tests and binding checks

• • Target concentration
• • Area response
• • Import
• • Binding check
• • Buffer autofluorescence

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Binding affinity assay

• • Target and ligand concentrations for binding assays
• • Affinity screening
• • Binder
• • Binding affinity
• • Import

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Advanced assays

• • Ternary interactions – overview
• • Assay setup for competitive binding measurements
• • Assay setup to measure the interaction between two unlabeled molecules using a fluorescent reporter molecule
• • Assay setup for cooperativity assessment (e.g. PROTACs, molecular glues)
• • Liquid handling

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