One of the most common ways to make a given target protein fluorescent is to attach organic Fluorophores by means of different labeling chemistries. NanoTemper Technologies provides different protein labeling kits with different labeling chemistries.

The available kits are:

Covalent chemistry:

• NHS-ester labeling for the covalent labeling of amines (mostly lysine residues).
• Maleimide reactive labeling for the covalent labeling of cysteine residues.
• SNAP®-Tag labeling kit.

Affinity-based labeling:

• tris-NTA labeling for the non-covalent labeling of His-tags. 
• Biotinylated target labeling kit.
• Human Fc labeling kit.

After the labeling reaction, we strongly recommend determining the Protein concentration and DOL. The DOL (degree of labeling) can very easily be calculated using the Degree-of-labeling Calculator.

Tip: For high throughput applications we strongly recommend using Large Volume kits to label target proteins in larger batches.