NanoDSF and backreflection
NanoDSF and backreflection
- • Unfolding profile
- • Transition vs. unfolding event
- • Unfolding profiles: redshift and blueshift
- • Ratiometric vs. single wavelength analysis
- • Antibody unfolding profiles
- • Turbidity parameters
- • How is the protein melting point (Tm) determined
- • Similarity of unfolding profiles
- • How onsets are determined in PR.Panta control
- • How are inflection points determined?
- • Fit models for thermal unfolding
- • Why are there unfolding profiles in different directions?
- • How to interpret nanoDSF data - ratio or single wavelength
- • How can I interpret the unfolding profile of an antibody?
- • Slope in the ratio before the main unfolding transition
- • What does the unfolding profile first derivative mean?
- • How are melting temperatures and onsets unfolding/aggregation determined on Prometheus NT.48 and NT.Plex
- • How is ΔG calculated by the PR.ChemControl software?
- • What does the starting value of the 350 nm/330 nm ratio mean?