PR.Panta Analysis offers the possibility to compare the similarity of unfolding profiles by assigning one sample as a reference. All other samples in the data set are then compared to the reference, and their profile similarity is output in %.

The similarity of two unfolding profiles is defined by the area between the curves.

For this, the area between the curves is calculated by looking at the difference in the ratio signals of the two curves at each temperature. Then, the area is corrected for the noise in the data, and the resulting corrected area Acorr is used to calculate the similarity value as follows:

As a consequence of this type of calculation, a small shift in the ratio (resulting in a shift of the profile along the y-axis) will result in a small change in similarity, whereas a shift of the inflection point or melting point (Tm) (resulting in a shift of the profile along the x-axis) will result in a comparably larger change in similarity.