Assay optimization
Assay optimization
- • Saturation
- • Initial fluorescence
- • Response amplitude
- • Random changes in initial fluorescence
- • Ligand-induced fluorescence change
- • Ligand-induced photobleaching
- • Signal quality
- • SD-test
- • ECP-test
- • Aggregation
- • Buffer screen
- • Aggregation quality
- • EC50
- • Failed wells
- • Ligand autofluorescence
- • Photobleaching
- • Quenching
- • Well-scan quality