Assay optimization

Assay optimization

• • [Video] Assay optimization tutorial
• • Ligand dependent initial fluorescence changes
• • Random changes in initial fluorescence
• • Assay buffer composition
• • Can I use TCEP in the assay buffer?
• • SD-test
• • ECP-test
• • Photobleaching in TRIC/MST experiments
• • Photoenhancement in TRIC/MST experiments
• • Viscosity in TRIC/MST assays