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Assay set up
Learn how to set up different types of assays on your Monolith
NanoTemper Technologies
Monolith
Assay set up
Target Labeling
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Properties of NanoTemper Technologies Dyes
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[Video] Target labeling tutorial
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Degree-of-labeling (DOL)
Pre-tests and binding checks
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[Video] Monolith pre-test and assay set up tutorial
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[Video] How to perform a pre-test in MO.Control tutorial
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Target Concentration
Binding affinity assay
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Protein-protein interactions with His-tag labeling
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[Video] How to perform a binding affinity experiment on MO.Control tutorial
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Target and ligand concentrations for binding assays
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Ligand serial dilution
Advanced assays
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Ternary Interactions – Overview
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Assay setup to measure the interaction between two unlabeled molecules using a fluorescent reporter molecule
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Assay setup for cooperativity assessment (e.g. PROTACs, molecular glues)
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Competitive Binding Assay
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Stoichiometry Experiment