Assay set up

Target Labeling

• • Properties of NanoTemper Technologies Dyes
• • [Video] Target labeling tutorial
• • Degree-of-labeling (DOL)

Pre-tests and binding checks

• • [Video] Monolith pre-test and assay set up tutorial
• • [Video] How to perform a pre-test in MO.Control tutorial
• • Target Concentration

Binding affinity assay

• • Protein-protein interactions with His-tag labeling
• • [Video] How to perform a binding affinity experiment on MO.Control tutorial
• • Target and ligand concentrations for binding assays
• • Ligand serial dilution

Advanced assays

• • Ternary Interactions – Overview
• • Assay setup to measure the interaction between two unlabeled molecules using a fluorescent reporter molecule
• • Assay setup for cooperativity assessment (e.g. PROTACs, molecular glues)
• • Competitive Binding Assay
• • Stoichiometry Experiment