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Critical Micelle Concentration (CMC) of LMNG protocol

Lauryl maltose-neopentyl glycol (LMNG) is a relatively new detergent which solubilizes and stabilizes a wide range of membrane proteins much better than traditional detergents. In this protocol, the critical micelle concentration (CMC) of LMNG is determined by measuring its interaction with a fluorescent dye. As soon as the concentration of LMNG is high enough to form micelles, the dye starts to be incorporated into them, which in turn leads to a more than two-fold fluorescence increase. In addition, dynamic light scattering measurements using the Prometheus Panta confirm the presence of micelles. The determination of the CMC value of surfactants under different environmental conditions is important for many different biological and chemical processes.
critical micelle concentration | LMNG

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