Reversible Quenching of Red Fluorophores by TCEP protocol
Tris(2-carboxyethyl)phosphine (TCEP) is a widely used reducing agent for the reduction of disulfide bonds on proteins. Some red fluorophores, including Cy5 and Alexa 647, are strongly quenched by TCEP. The quenching is caused by a reversible addition of TCEP to the dye with a dissociation constant in the mM range. To avoid interference in Monolith binding assays, TCEP should only be used at sub-mM concentrations.
small molecule – small molecule | quenching