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Affibody – IgM-Fc (stoichiometry) protocol

Affibodies are small (~6 kDa) recombinant proteins which bind in a monovalent fashion to their target with high specificity and affinity. The affibody used here binds to membrane-associated IgM-BCRs (mIgM-BCR). The B cell antigen receptor (BCR) consists of a plasma membrane–bound antibody that is associated with a pair of signaling proteins. Antigen binding to the BCR stimulates B cells to differentiate into antibody-secreting cells. Since mIgM-BCRs are arranged as homodimers, it is expected that two affibodies bind to a single IgM molecule.

protein – protein interaction | affibody | IgM | stoichiometry | MO-P-050

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