Streptavidin (SA) is a homo-tetramer that has an extraordinarily high affinity for biotin (also known as vitamin B7). It is used extensively in molecular biology and bio-nanotechnology due to the streptavidin-biotin complex's resistance to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH. MST can be used to determine the stoichiometry of the biotin-streptavidin interaction.

protein – small molecule interaction | streptavidin | stoichiometry | MO-P-042