EcoSSB (E. coli single-stranded DNA binding protein) plays a key role during DNA replication by binding single-stranded DNA (ssDNA), thereby preventing re-annealing of the strands and allowing replication by DNA primases and polymerases. Biophysical in vitro and crystallographic studies showed that one EcoSSB tetramer binds a single ssDNA oligonucleotide over a total length of 70 nucleotides.

protein – DNA interaction | DNA replication | MO-P-035