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Protein A – Herceptin (with cystine labeling) protocol

 

Protein A is a cell surface receptor found in the cell wall of the bacteria Staphylococcus aureus. It’s frequent use in biochemical research derives from its ability to bind the Fc region of most immunoglobulins. In this protocol, Herceptin (Trastuzumab), a monoclonal antibody used to treat breast cancer, is labeled site-specifically after selective reduction of antibody interchain disulfides with a maleimide-dye.

protein – protein interaction | antibody | antibody labeling | MO-P-012

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