Abstract

Antibody-drug conjugates (ADCs) have been recognized as a promising new class of therapeutic agents for the treatment of cancer (1). In the generation of ADCs, cytotoxic small molecules are covalently attached to therapeutic antibodies thereby increasing their tumor cell killing capability. We have performed MicroScale Thermophoresis experiments to determine the Kd values for the interaction between fluorescently labeled antibody-drug conjugate (SYD985) and an anti-toxin (drug) antibody in phosphate buffer as well in human plasma. The data show that MicroScale Thermophoresis is a powerful tool for the study of antibody-antigen interactions in standard buffers as well as complex bioliquids.