Optical Unfolding Analysis is a cloud-based application available on the NanoTemper Hub. It is designed to help scientists further evaluate Optical Unfolding data generated on Dianthus α, giving researchers a dedicated analytical environment to assess protein thermal stability and characterize binding interactions under near-native conditions.

What Does the Optical Unfolding Analysis App Do?

The Optical Unfolding Analysis application on NanoTemper Hub ingests raw Optical Unfolding data from Dianthus α, processes the unfolding curves, and delivers structured analytical outputs tailored to the following applications:

• Protein quality assessment — for verifying that your protein is properly folded and stable before starting binding experiments

• Binding validation — for confirming ligand binding by detecting ligand-induced stabilization, orthogonal to affinity measurements

• Identifying problematic compounds — for flagging aggregators and auto-fluorescent compounds that may produce artifacts in your assay

• Characterizing multi-component systems — for studying complex assemblies such as binary and ternary complexes, including PROTAC-mediated interactions

A detailed guide on the App usage can be found in Software Workflow - Optical Unfolding.

How to Access the Optical Unfolding Analysis App

Optical Unfolding Analysis access is available for Dianthus α instruments with the Optical Unfolding feature enabled. For more information about purchase options for Optical Unfolding, please contact our specialists.

If you have already acquired the Optical Unfolding feature and require access to Optical Unfolding Analysis on the NanoTemper Hub, please reach out to us at support@nanotempertech.com and state the device's serial number (e.g. DI-00123).