The excitation power is the strength of the LED used for excitation of Fluorophores. In DI.Control the Excitation Power, which can be set to Auto-Excitation or a manual setting. In the Auto-Excitation mode, the Dianthus instrument determines the best Excitation Power setting based on the sample in the first well that is to be measured (Dianthus tries to adjust the excitation power to reach an Initial Fluorescence count of 12.500 FU). That means one should be careful using this setting if one expects significantly different sample brightnesses within one experiment. 

When using a manual setting in contrast to an automatic one, adjust the excitation power to ensure your sample's Fluorescence signal is within the required range. An ideal value is between 10000 and 14000 fluorescence counts. To ensure comparability of data, one should measure data to be compared with the same excitation power.

Note: The minimum fluorescence count required for Dianthus measurements is 1000, while the detector reaches saturation at 24999 fluorescence counts.