For NanoTemper Technologies assays, the target is the fluorescent biomolecule, which binds to the ligand. The concentration of target is kept constant throughout all the Serial Dilution, while the concentration of non-fluorescent Ligand is varied.

Dianthus measurements with Spectral Shift and TRIC are not restricted to a specific type of target molecule. They can be applied to proteins such as Antibodies, Enzymes, GPCRs, Membrane Proteins, IDPs, etc., but also to research Nucleic Acids and other biologically relevant molecules.