In some cases, DI.Screening Analysis can single out problematic wells in affinity measurements (e.g. an outlier in Initial Fluorescence, in Photobleaching, Ratio for Spectral Shift, or Fnorm for TRIC measurements) and excludes them from analysis (not from view in charts). The software uses Quality Checks to flag outliers, which can, however, be overridden by the user. In DI.Screening Analysis, the maximum number of outliers flagged in one affinity measurement depends on the number of data points measured. This can also serve as a general recommendation for the exclusion of data points from affinity measurements:

*for each increment of 8 datapoints, one more flagged outlier would be allowed per category.

Note:  The outlier rating might differ depending on whether you are working with Merge Sets or individual replicates due to the global fit.