In a single-dose screening, a ligand is considered a non-binder if it yields results similar to those of the reference sample. In Affinity Screening a ligand is considered a non-binder if the Dose Response Curve reaches less than 25% Saturation as determined by the fit (Kd Model or Hill Model). In general, for Single-Dose and Affinity Screenings, the Response Amplitude will determine if DI.Screening Analysis considers a ligand a non-binder or assigns it a different Ligand Category.