The NanoTemper Technologies Software products DI.Control and DI.Screening Analysis are both able to automatically detect issues with assay quality, such as Initial Fluorescence, and use Quality Checks to make the user aware of them. They usually have an automatically assigned negative or positive value, depending on the sample quality, which can be overridden by the user. For initial fluorescence data, the software checks for too high or too low fluorescence values, Random Changes in Initial Fluorescence, and Ligand-Induced Fluorescence Change. It is possible that the fluorescence quality is set to inconclusive. This can for example be the case if different wells corresponding to the same ligand show inconsistent initial fluorescence data. In single-dose screening a ligand-induced fluorescence change is detected if the initial fluorescence values of a ligand deviate by more than ± 20 % from the respective references. In affinity screening a ligand-induced fluorescence change is detected if the initial fluorescence values for the labeled target or tracer molecule display a clear ligand-induced change.