Most used biological buffers don’t show a significant fluorescence signal at the red emission wavelengths, which is relevant for Dianthus experiments. Some substances, however, can. To test for buffer autofluorescence, load the buffer alone into an empty well and measure the well in DI.Control using the Quick Start Single-dose template while setting the Excitation Power manually to 100 %. Buffer autofluorescence is considered problematic if it is 50% higher than the fluorescence of the labeled target molecule.

One option to address buffer autofluorescence is to change to a non-fluorescent buffer or to remove the fluorescent buffer component. Increasing the target concentration may also help, but it is generally not the recommended solution for this issue.