Maltose binding protein (MBP) is part of the periplasmic transport system of Escherichia coli and involved in the transport of maltose into the bacterium. It binds the disaccharide once it has crossed the outer membrane, and then assists its translocation across the inner membrane. Additionally, it is often used as a fusion tag for protein purification or solubilization. In this protocol, biotinylated MBP with an AVI-tag is fluorescently labeled using Nanotemper Technologies’ Biotinylated Target Labeling Kit (NT-L020).

protein – small molecule | carbohydrate | conformational change | MOX-P-105