In PR.Panta Control software, all measurements performed on a single set of capillaries are grouped together in an experiment. Measurements can be added and executed consecutively by choosing from the available measurement types below.

Size Analysis Dynamic Light Scattering (DLS) measurement performed at a single temperature to determine particle sizes and assess sample homogeneity. The main output parameters are the hydrodynamic radius, the polydispersity index (PDI), and the size distribution.

Note: Results from a Size Analysis measurement can also be used to calculate the diffusion interaction parameter kD and B22/MW.

Thermal Unfolding Applies a linear thermal ramp to samples while reading out fluorescence and turbidity to analyze thermal stability and aggregation behavior. Scattering readout from the DLS optics can optionally be enabled in parallel to provide a more in-depth view of the aggregation process. The main outputs are plots of the respective signals over temperature, from which inflection points and onset temperatures can be determined.

Isothermal Scan Monitors sample fluorescence and aggregation over time at a constant, user-defined temperature. This mode is well suited for assessing time-dependent stability and real-time aggregation behavior.

Chemical Unfolding Uses chemical denaturants such as urea or guanidinium chloride to measure protein unfolding rapidly and efficiently. The software automatically fits the data to extract key thermodynamic parameters, including the free energy of unfolding (Delta G) and c50, the denaturant concentration at which 50% of the protein is unfolded.

Temperature Control Gives you more flexibility over the thermal ramp. With temperature cycling, program heating and cooling cycles to identify constructs or conditions for reversibility of protein unfolding at the domain level. With temperature stepping, model process conditions with defined temperature holds. These modes can be unlocked for Prometheus Panta through a separate software activation process. For more information, please contact our specialists.