Thermal unfolding allows for application of a linear thermal ramp to the sample while reading out fluorescence and turbidity. Reading out scattering from the DLS optics in parallel is optional. Carry out the following steps before starting a measurement in PR.Panta Control:

• Set the excitation power. The value for the excitation power is automatically carried over from the last selected discovery scan. You can modify it manually between 1-100%.
• Set the temperature slope (°C/min) to the desired heating rate.
• Define start and end temperature.
• Activate Refolding Ramp to include a refolding measurement with a temperature slope identical to the unfolding measurement, if desired. 
• Select to run thermal unfolding with or without dynamic light scattering (DLS). With DLS set to ON, Prometheus Panta will continuously cycle through all capillaries and records a single acquisition of 500 ms per capillary in each round. DLS laser power and solvent (along with its viscosity) only need to be specified when Dynamic Light Scattering is set to ON.
• Set the DLS laser power, if applicable. The value for the DLS laser power is automatically carried over from the last selected discovery scan. You can modify the laser power manually between 1-100%
• In the annotations table, select a solvent (along with its viscosity) for each capillary. The viscosity is required to calculate the hydrodynamic radius.
• Add annotations. Additional columns can be added via the button. Annotations and annotation columns can also be modified via the same dialogue in the data table after the measurement is completed.

Note: Acquiring DLS data during a thermal unfolding measurement reduces the datapoint density of the fluorescence and turbidity signals. A warning symbol indicates if onset and inflection point calculation may be compromised.