Excitation power

The excitation power is the intensity of the UV-LED used for the excitation of fluorophores, in this case the aromatic amino acids tryptophan and tyrosine. In PR.Panta Control the optimal excitation power for an experiment is set during a Discovery scan. The excitation power setting of the selected discovery scan will be automatically carried over to the measurement settings but can be manually changed during setup of each measurement.

Note: Photobleaching effects are negligible even at high excitation intensities due to the rapid on-the-fly measurement mode.

Note: If the capillary fluorescence exceeds the detector limit during an unfolding experiment, the corresponding data points will not be displayed and will be excluded from data analysis.

DLS laser power

The DLS laser power is the intensity of the DLS laser used for dynamic light scattering measurements. In PR.Panta Control the optimal laser power for an experiment is set during a Discovery Scan. The laser power setting of the selected discovery scan will be automatically carried over to the measurement settings, but can be manually changed during setup of each measurement.

Note: 100% DLS laser power is usually the most appropriate setting, because higher light intensity results in less noise. DLS laser power should only be reduced if the discovery scan shows detector saturation.

Note: The fluorescence intensity is used to determine the optimal position for the DLS optics for each scan cycle and DLS data quality may suffer if fluorescence intensities are below the measurement limit. Even if you plan to only measure DLS, make sure that the fluorescence intensity of the capillaries is sufficiently high.