Reliable calculation of inflection points and onsets in thermal unfolding measurements, requires sufficient datapoint density. When running thermal unfolding measurements without DLS on Prometheus Panta, the datapoint density will be high because acquiring a nanoDSF and turbidity datapoint for all 48 samples takes about 3 seconds.

When performing a thermal unfolding measurement with dynamic light scattering, one DLS acquisition is made per capillary for every two nanoDSF and turbidity datapoints. To acquire a DLS datapoint, the tray needs to stop for each capillary for the duration of the acquisition (500 ms), thus reducing the achievable datapoint density in all channels.

In PR.Panta Control the expected datapoint density is displayed during measurement setup and a notification is given to the user: A checkmarkwill appear for datapoint densities of ≥1.9 points/°C, which is sufficient for all types of samples. The warning iconwill appear for <1.9 points/°C, indicating settings yielding insufficient density.

The datapoint density can be increased either by reducing the thermal ramp or the number of capillaries measured. It is still possible to use the results of a measurement with insufficient data density but please be aware that the inflection points and onsets may be less reliable and less reproducible.