As a lot of different proteins coexist in cells, it is important to understand how an aggregating protein can affect the aggregation of another protein in its vicinity. Amyloid formation is typically monitored in Thioflavin T (ThT) assays, although the exact mechanism of action and whether the dye itself affects the kinetics of aggregation is still debated. In this protocol, the in vitro coaggregation and cross-seeding of lysozyme and bovine serum albumin (also known as BSA) during their amyloid formation is followed in real time without the need of a dye. L-Arginine is one of the most commonly used and most generally applicable suppressors of protein aggregation.

coaggregation | cross-seeding | amyloid formation | aggregation suppression | PR-P-017