Major Histocompatibility Complex class I (MHC class I) molecules present variable and non-self antigenic peptides to CD8⁺ T cells. In this way, they play a very significant role in our body’s immune defense mechanisms against pathogens and tumors. MHC class I molecules are used to generate MHC tetramers that have wide applications in T cell staining, isolation, and activation. In immunotherapy, MHC tetramers are used to trace T cells in patients' blood. Hence, these MHC class I molecules are popular topics of research. Biochemical characterization of these proteins involves studies on structural stability, peptide binding affinity, pH stability, and storage conditions. The thermal denaturation method can be used to answer all these parameters.

thermal unfolding | T_m | MHC class I | PR-P-007