DNA Aptamer – Ochratoxin (in milk) protocol
DNA aptamers are molecules that can be produced with high quality and low batch-to-batch variations via chemical synthesis. In addition, they bind their targets with high affinity. In the field of biosensor development, aptamers are a valuable alternative to antibodies in detecting small molecules. In this protocol the binding of an aptamer to ochratoxin is analyzed. Ochratoxin A is a mycotoxin found in food. It is nephrotoxic and carcinogenic in the kidney and induces differentiation in cloned renal cell lines. The binding is assayed in buffer, as well as in a buffer spiked with milk.
aptamer – small molecule interaction | complex bioliquid | MO-P-057