Protein A – Rabbit & Mouse IgG protocol
Protein A is a cell wall component produced by several strains of Staphylococcus aureus. The protein A molecule contains four high affinity binding sites capable of interacting with the Fc region of IgG from several species, including rabbit. Conversely, protein A has a much lower affinity towards mouse IgGs. Given the high affinity of protein A to numerous IgGs, it is commonly used in biochemical research for immunoassays such as Western blotting, immunohistochemistry and ELISA.
protein – protein interaction | antibody | MO-P-040