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Protein A – Rabbit & Mouse IgG protocol

Protein A is a cell wall component produced by several strains of Staphylococcus aureus. The protein A molecule contains four high affinity binding sites capable of interacting with the Fc region of IgG from several species, including rabbit. Conversely, protein A has a much lower affinity towards mouse IgGs. Given the high affinity of protein A to numerous IgGs, it is commonly used in biochemical research for immunoassays such as Western blotting, immunohistochemistry and ELISA.

protein – protein interaction | antibody | MO-P-040

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