Logo

DNA Aptamer – AMP (displacement assay) protocol

The DNA aptamer for adenosine is a highly conserved sequence that is a widely used model aptamer for biosensor development. It also binds ADP and ATP, and with slightly weaker affinity AMP. The affinity between unlabeled aptamer and AMP can be determined by a displacement assay with a short, fluorescently labeled ssDNA (cDNA) complementary to parts of the aptamer sequence.

DNA – small molecule interaction | aptamer | displacement assay | MO-P-037

Was this article helpful?