CMB3a – Cellulose Nanocrystals protocol
The family 3 carbohydrate-binding module CBM3 is part of the scaffolding subunit of the multienzyme plant cell wall degrading complex termed cellulosome. Cellulosomes are anchored to the external membrane of some lignocellulolytic microorganisms. Several hydrolytic enzymes active on plant polysaccharides are grafted to the cellulosome. However, the CBM3a is a non-catalytic protein able to bind to insoluble cellulose. Its main functionality is to bring the cellulosome in close vicinity to the plant cell wall and enhance the deconstruction of the plant polysaccharides as a source of carbon for the microorganism.
protein – carbohydrate interaction | cellulose | nanocrystals | MO-P-031