30S Ribosomal Subunit – YjeQ protocol
The ribosome is responsible for translating the genetic code into functional proteins and is often a target for antimicrobial research. Specifically, the 30S ribosomal subunit decodes mRNA to ensure high fidelity during protein synthesis. Although much is known about protein translation, the details surrounding ribosome biogenesis remain largely elusive. Ribosome biogenesis is a complex process that requires the assembly of multiple proteins and rRNA molecules to form a functional 70S ribosome in bacteria. This process is aided by multiple assembly factors that transiently interact with assembling ribosome particles to aid in their maturation. A central protein involved in this process is the GTPase YjeQ, which has been associated with aiding in the later stages of 30S maturation.
protein – protein interaction | ribosome assembly | GTPase | biogenesis | MO-P-027