BoxP1 Hairpin DNA – Mlc protocol
Mlc (makes large colonies) from Escherichia coli is a transcriptional repressor which controls the expression of a number of genes encoding enzymes involved in glucose metabolism. Mlc forms stable homodimers in order to bind DNA, though it usually is found as a tetramer in studies using size exclusion chromatography. The hairpin DNA is a model for the operator sequence that Mlc interacts with. This interaction has been characterized with SPR and ITC as well.
protein – DNA interaction | gene regulation | MO-P-024