Bovine Serum Albumin Self-Oligomerization protocol
Bovine serum albumin (also known as BSA) is a serum albumin protein derived from cows. It is often used as a protein concentration standard in lab experiments. At high concentrations, it undergoes a completely reversible self-oligomerization and subsequent structural alteration. Upon dilution, the oligomers dissociate again into a native monomeric state. Polysorbate 20 (TWEEN® 20) can increase the colloidal stability of proteins by interacting with the hydrophobic surface areas of proteins in order to minimize protein-protein interactions and thus protein aggregation.
self-oligomerization | beta-sheet formation | colloidal stability | MO-P-019