NanoTemper® Technologies’ PrometheusTM instruments detect changes in the fluorescence emission of fluorescent amino acids upon unfolding using nanoDSFTM, an advanced Differential Scanning Fluorimetry technology. The instruments can be used to induce thermal unfolding of proteins and to determine thermal unfolding transition temperatures. Furthermore, the instruments are equipped to investigate chemical unfolding and the free energy of unfolding ΔG in an extraordinarily straightforward and fast manner.

The PR.ChemControl software is dedicated to running and analyzing chemical unfolding (and optionally also refolding) experiments on Prometheus instruments.