Binding of Calcium Ions to Synaptotagmin
Abstract
The synaptic vesicle protein synaptotagmin 1 is the main calcium sensor of neuronal exocytosis. Calcium binds to its cytosolic portion that consists of tandem C2-type domains. In this work we show that MicroScale Thermophoresis is a valuable tool to measure binding of ions to proteins, with and without the use of a fluorescent label.