Abstract

Self-interaction of therapeutic proteins has an impact on the ultimate success of a biologic candidate. During the development process, candidates are often assayed at concentrations that differ from that of their final delivery form. Understanding how therapeutic proteins behave at higher concentrations is crucial for ensuring their ultimate success in the clinic. Dynamic (DLS) and static (SLS) light scattering techniques help researchers determine and optimize the self-interaction behavior of protein-based therapeutics ahead of scale-up and manufacture. Here we show how the Prometheus Panta measures self-interaction of proteins and allows researchers to prioritize candidates and improve outcomes.